Caenorhabditis elegans DYF-2, an orthologue of human WDR19, is a component of the intraflagellar transport machinery in sensory cilia.

Intraflagellar transport (IFT) machinery needed to build functional cilia consists of a multisubunit complex whose molecular composition, organization and functions are poorly understood. Here, we describe the tryptophan-aspartic acid (WD) repeat Novel (WDR) containing IFT protein of Caenorhabditis elegans, DYF-2, which plays an important role in maintaining the structural and functional integrity of the machine IFT. 

We determine the identity dyf-2 gene by transgenic rescue mutant Human Clia kits phenotype and by sequencing the mutant allele. Losing DYF-2 selective function affects the assembly and motility of different IFT components and leads to defects in cilia structure and chemosensation in nematodes. 

Based on these observations, and analysis DYF-2 moves in Bardet-Biedl syndrome mutant with partially disrupted IFT particles, we conclude that the DYF-2 can associate with IFT complex particles B. At the same time, mutation in dyf-2 can interfere with the function of the component A complex, suggesting an important role of this protein in the overall assembly of IFT particles. Importantly, the mouse orthologue DYF-2, WDR19, also localizes to cilia, pointing to an important evolutionarily conserved role for this WDR protein in cilia development and function.
Caenorhabditis elegans DYF-2, an orthologue of human WDR19, is a component of the intraflagellar transport machinery in sensory cilia.

mastic oil of Pistacia lentiscus var. growth and survival of human K562 leukemia cells and attenuates chia inhibit angiogenesis.


mastic oil of Pistacia lentiscus var. chia, natural plant extracts traditionally used as a food additive, has been studied extensively for antimicrobial activity associated with the combination of its bioactive components. One of them, perillyl alcohol (POH), chemopreventive featuring the tumor, chemotherapy and antiangiogenic properties. We investigated whether mastic oil will also suppress tumor cell growth and angiogenesis. 

We observed that the mastic oil concentration and time dependence given antiproliferative and proapoptotic effects on human leukemia K562 cells and inhibit the release of vascular endothelial growth factor (VEGF) on K562 and B16 mouse melanoma cells. In addition, the mastic oil caused a concentration-dependent inhibition of endothelial cell (EC) proliferation without affecting cell viability and a significant decrease microvessel formation both in vitro gentaur-antibodies.com and in vivo. 

Investigating the mechanisms underlying (s) indicates that the oil is reduced mastic 1) in K562 cells signal-regulated kinase activation of extracellular 1/2 (Erk1 / 2) are known to control leukemia cell proliferation, survival, and VEGF secretion and 2) in EC activation RhoA, an important regulator of the organization neovessel

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